Zeno SWATH Data Independent Acquisition (DIA)
SWATH
Dive deeper into the biomarker landscape with Zeno SWATH Data Independent Acquisition (DIA).
SWATH DIA (information independent acquisition) has actually emerged as an LC-MS/MS workflow that capitalizes on the speed and sensitivity gains that have actually been accomplished with contemporary mass spectrometers. SWATH DIA can supply recognition of large varieties of proteins, peptides and metabolites in a more reproducible manner than standard data-dependent acquisition (DDA) approaches. With SWATH DIA, premium, high-resolution MS/MS data not only supply identification but are also the secret behind exact and highly accurate metrology.
Now, Zeno SWATH DIA marks a significant step-change in information independent acquisition delivering a high depth of protection, especially on low abundance species, rapidly and robustly.
Exclusively on the ZenoTOF 7600 system from SCIEX, the Zeno trap innovation has expanded the reach of assays for numerous applications through sensitivity acquires provided through MS/MS responsibility cycle enhancements.
With the Zeno trap, nearly > 90% duty cycles are now attainable for all ions no matter lc, charge, or mass flow rate. This provides level of sensitivity gains of 4- to 20-fold without jeopardizing other important performance specifications such as resolution, mass precision, dynamic variety and speed.
The Zeno SWATH DIA workflow now profits from these gains to make it possible for thorough and genuinely prevalent analyte protection at brand-new depths of detection and higher throughput. With Zeno SWATH DIA, greater sensitivity reveals more lower-level analytes, allows quicker and more robust chromatography and the high-end of utilizing much lower sample amounts.
Uncover the depth of the proteome.
SWATH DIA has actually ended up being the approach of option for massive proteomics and other research studies. The sensitivity gains that Zeno SWATH DIA offers widen the application area to include regularly performing these research studies using microflow, and even high-flow, chromatography for more robust and rapid analyses.
Greater sensitivity likewise equates to higher quality information for lower levels of analyte. Nanoflow chromatography Zeno SWATH DIA supplies reproducible qualitative and quantitative data at sample levels approaching single-cell analysis. With single-cell analysis, discrete cellular states and occasions can be separated from bulk proteomic discoveries, resulting in a better understanding of cellular heterogeneity. Reproducible and precise quantification at these levels is crucial, however it is typically more challenging or labor-intensive to acquire these abundance measurements from profiling information or labeling strategies. Zeno SWATH DIA quantification utilizing MS/MS information prevents numerous issues by offering higher intensity spectra and high MS/MS scan rates with a broad dynamic variety.
Optimum details from each valuable sample.
Zeno SWATH DIA combines the sensitivity of the Zeno trap with the reproducibility and accuracy of SWATH DIA to provide unprecedented levels of analyte recognition and quantification. The 6-10x level of sensitivity gains in MS/MS mode that the Zeno trap supplies through duty cycle enhancements deliver up to 3x more identified proteins, and ~ 3-6x more measured, at loads less than 20 ng. This causes a more comprehensive understanding of underlying biological changes.
- Identify and measure approximately 2x as lots of proteins just like standard SWATH DIA methods.
- Shorten runtimes to just 5 minutes with minimal compromise in proteome protection.
- Decrease sample size to a tenth or twentieth with low loads of 10 ng.
With SWATH DIA, premium, high-resolution MS/MS data not just offer recognition however are also the trick behind extremely accurate and exact quantification.
Nanoflow chromatography Zeno SWATH DIA offers reproducible qualitative and quantitative data at sample levels approaching single-cell analysis. Zeno SWATH DIA metrology using MS/MS information circumvents many problems by providing greater strength spectra and high MS/MS scan rates with a large dynamic variety.
Zeno SWATH DIA integrates the level of sensitivity of the Zeno trap with the reproducibility and accuracy of SWATH DIA to provide extraordinary levels of analyte recognition and metrology.
